用于辅助检测大豆百粒重的引物和检测方法

Primer for auxiliary detection of soybean hundred-grain weight, and detection method thereof

Abstract

本发明公开了一种用于辅助检测大豆百粒重的引物,包括如下一对引物:F:CGCCTTACTCTGTATCTTCT;R:CACCTCTTCCTCACTCCT。本发明还提供了一种检测大豆百粒重的方法,包括以下步骤:以待测大豆基因组DNA 为模板,将上述引物对上述DNA模板进行PCR 扩增;PCR扩增产物进行酶切,检测酶切产物中是否有238bp和181bp两条带,若酶切产物中有238bp和181bp两条带,上述待测大豆为大籽粒,若扩增产物中无238bp和181bp两条带,上述待测大豆为小籽粒。本发明的优点在于,可以通过对育种材料进行筛选,淘汰无238bp和181bp目标条带的亲本或育种中间材料,极大的提高了育种过程中选择高百粒重大豆品种的效率,对于高产大豆品种的培育效果显著。
The invention discloses a primer for the auxiliary detection of the soybean hundred-grain weight. The primer comprises a pair of primers comprising F having a sequence represented by CGCCTTACTCTGTATCTTCT and R having a sequence represented by CACCTCTTCCTCACTCCT. The invention also provides a method for detecting the soybean hundred-grain weight. The method comprises the following steps: carrying out PCR amplification of a template which is genome DNA of soybean for detecting by the above primer pair; and carrying out enzyme digestion of PCR amplification products, detecting whether there are 238bp and 181bp bands in enzyme digestion products, determining the soybean for detecting being a large grain if so, and determining the soybean for detecting being a small grain if not. In the invention, a parent or breeding intermediate material having no 238bp or 181bp target bands can be eliminated through screening breeding materials, so the selection efficiency of the high-hundred-grain-weight soybean kind is greatly increased, and the cultivation effect of the high-yield soybean kind is substantial.

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Patent Citations (2)

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Cited By (2)

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    CN-104894123-ASeptember 09, 2015中国农业科学院作物科学研究所Method for assisted identification of pod bearing habit of glycine max and special primers for method
    CN-105087757-ANovember 25, 2015河北省农林科学院粮油作物研究所Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker